Synthesis and characterization of Sophora alopecuroides L. green synthesized of Ag nanoparticles for the antioxidant, antimicrobial and DNA damage prevention activity

Abstract In this study, it was aimed to investigate the amount of antioxidant, protective properties against DNA damage and antibacterial properties against various pathogens after the interaction of Ag metal (Ag NPs/Sa) of Sophora alopecuroides L. (S. alopecuroides L) plant seed, which is grown in Igdir and used in the treatment of many diseases. The DPPH radical quenching activity of Ag NPs/Sa was performed by using Blois method, DNA damage prevention activity by gel electrophoresis and antibacterial property by disk diffusion method. With the green synthesis method, AgNPs obtained as a result of the reaction of the plant and Ag metal are UV visible spectrophotometer (UV-vis), fourier-transformed infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscope (SEM). DPPH radical quenching activity of Ag NPs/Sa was investigated in the concentration range of 25-250 µg/ml. The radical quenching activity at a concentration of 250 µg/ml was 85,215 ± 0,101%, while this value was 93,018% for the positive control BHA. It has been observed that the protective property of pBR322 plasmid DNA damage against OH radicals originating from H2O2 increases with concentration. It has been observed that Ag NPs/Sa has significant antimicrobial properties against some pathogens (B. subtilis ATCC 6633 E. coli ATCC 25952, B. cereus ATCC 10876, P. aeruginosa ATCC 27853, E. faecalis ATCC 29212, S. aureus ATTC 29213 and C. albicans ATTC 90028) that cause disease and even some pathogens are more effective than antibiotics

Aryl sulfonate based anticancer alkylating agents

This research work revolves around synthesis of antineoplastic alkylating sulfonate esters with dual alkylating sites for crosslinking of the DNA strands. These molecules were evaluated as potential antineoplastic cross linking alkylating agents by reaction with the nucleoside of Guanine DNA nucleobase at both ends of the synthesized molecule. Synthesis of the alkylating molecules and the crosslinking with the guanosine nucleoside was monitored by MALDITOF mass spectroscopy. The synthesized molecule's crosslinking or adduct forming rate with the nucleoside was compared with that of 1,4 butane disulfonate [busulfan], in form of time taken for the appearance of [M+H]+ . It was found that aryl sulfonate leaving group was causing higher rate of nucleophilic attack by the Lewis basic site of the nucleobase. Furthermore, the rate was also found to be a function of electron withdrawing or donating nature of the substituent on the aryl ring. Compound with strong electron withdrawing substituent on the para position of the ring reacted fastest. Hence, new alkylating agents were synthesized with optimized or desired reactivity

Do submucous myoma characteristics affect fertility and menstrual outcomes in patients underwent hysteroscopic myomectomy?

Submucous myomas may be associated with menorrhagia, infertility and dysmenorrhea. The aim of this study was to determine the long term effects of submucousal myoma resection on menorrhagia and infertility; also to detect whether the type, size, and location of myoma affect the surgical success. Totally 98 women referred to hysteroscopy for symptomatic submucousal fibroids [menorrhagia [n=51] and infertility [n=47]] between 2005- 2010 were enrolled in this historical cohort study Pregnancy rates and menstrual improvement rates were compared according to myoma characteristics [size, type and location]. After a mean postoperative period of 23 +/- 10 months in 51 patients with excessive bleeding, 13 had recurrent menorrhagia [25%]. In Other 38 patients excessive bleeding was improved [75%]. The improvement rates by location and myoma type: lower segment 100%, fundus 92%, and corpus 63%; type 0] 70%, type 1] 78%, type 2] 80%. The mean sizes of myoma in recurred and improved patients were 23.33 mm and 29.88 mm respectively. 28 of 47 infertile women spontaneously experienced thirty pregnancies [60%]. Pregnancy rates according to myoma location and type: lower segment 50%, fundus 57%, and corpus 80%; type 0] 75%, type 1] 62%, type 2] 50%. The mean myoma size in patients who became pregnant was 30.38 mm; in patients who did not conceive was 29.95 mm. The myoma eharacetesitics do not affect improvement rates after hysteroseopie_myomectomy in patients with unexplained infertility or excessive uterine bleeding

Functional evaluation of partial hip arthroplasty in patients with hip fractures

The goal of this study was to evaluate the functional results of partial hip arthroplasty in hip fractures. The mortality, morbidity, and functional statuses were investigated. Patients operated with partial hip arthroplasty for the hip fractures between January 3, 2001, and January 30, 2010, were evaluated retrospectively. The Oxford hip score was carried out, and the results were analyzed.A total of 82 of 130 patients who were operated with partial hip arthroplasty for hip fractures reached Fatih University Hospital [new name of Turgut Ozal University]. The mean follow-up time was 37.76 months and the mean age of the 82 patients was 78.99. Of the total number of patients, 25 were men and 57 were women. A total of 49 patients were alive and 33 were dead. The mean postoperative survival of these patients was 31.1 months. The mean total score of the Oxford hip score was 50.17. There was no effect of operation age, sex, hypertension, diabetes mellitus, renal diseases, dementia, and chronic obstructive lung disease on the Oxford hip score. Survival was not affected by the result of the Oxford hip score. The total Oxford hip score was less in patients who had heart disease, but it was not significant statistically. Embolus affected the hip score negatively. Partial hip arthroplasty takes a big place in the treatment of hip fractures, and it is a successful treatment choice in functional evaluation. New investigations are needed to compare the functional results of partial hip arthroplasty with other treatments

Predictive factors influencing pregnancy rates after intrauterine insemination

So far, many studies investigated factors that affect pregnancy rates after intrauterine insemination [IUI]. Various investigators have not agreed on the nature and ranking of these criteria. The aim of this study was to assess the predictive factors for pregnancy rate after controlled ovarian hyperstimulation [COH]/ IUI. Retrospective study of all patients undergoing IUI at Zeynep Kamil Gynecologic and Pediatric Training and Research Hospital from January 2006 to December 2009. In total 980 IUI cycles in 569 couples were analyzed. All women in the study underwent ovarian stimulation using gonadotropin and IUI was performed 36 h after triggering ovulation. The primary outcome measure was clinical pregnancy rates. Predictive factors evaluated were female age, body mass index [BMI], duration of infertility, type of infertility, follicle stimulating hormone [FSH] level and estradiol [E[2]] on third day of the cycle, number of preovulatory follicles, endometrial thichness, total motil sperm [TMS] count, and ratio of progressive motile sperm. The overall clinical pregnancy rate was 4.7%. Among the predictive factors after multivariate logistic regression analysis level of BMI [<25 kg/m[2]], number of preovulatory follicles [> =2], level of FSH [<9.4 IU/L], level of E2 [<80 pg/ml] and the ratio of progressive motile sperm [>50%] significantly influenced the clinical pregnancy rate. Level of BMI, FSH, estradiol, number of preovulatory follicles and the ratio of progressive motile sperm may determine IUI procedure as optimum treatment model

Fertility sparing surgery on placenta invasion anomalies and placenta previa

Abnormal placental invasion has increased parallel with persistent rise in Caesarean delivery. Management relies on accurate diagnosis and delivery should be planned at an institution with appropriate expertise and resources for managing this condition. We present a case of a placenta invasion anomaly which is the major risk factors of peripartum deaths. In this case we try to explain our approach which reduces unnecessary hysterectomy rates. In order to avoid postpartum hemorrhage and hysterectomy protocols, our approach which consists bilateral hypogastric arterial ligation, Bakri balloon tamponade and if necessary methotrexate therapy can be applied successfully

Clinical features and early diagnosis of typhoid fever emphasizing usefulness of detecting mesenteric lymphadenopathy with ultrasound as diagnostic method.

Typhoid fever is endemic in Pakistan. Most patients are children. As the symptoms and signs are often unspecific, it is difficult to diagnose typhoid fever without blood culture. We retrospectively reviewed 51 cases of typhoid fever who were all admitted from 1 June through 31 August 2002. Sixteen cases were positive by blood culture and confirmed as typhoid fever. All cases had Salmonella typhi. Although 16 cases were culture-negative, they were clinically diagnosed as typhoid fever. The remaining 19 cases were clinically diagnosed without blood culture. The clinical features of the culture-confirmed cases were more severe than the culture-negative cases. Mesenteric lymphadenopathy was very frequently detected with ultrasonography, in both culture-confirmed and culture-negative cases. The rates of detecting mesenteric lymphadenopathy were 69% and 63%, respectively. Meanwhile, the rate in non-typhoid fever patients was 5.5%. It was considered that detecting mesenteric lymphadenopathy with ultrasonography was very useful in the diagosis of typhoid fever in endemic areas.